Southend United lost 3-0 to Premier League new boys Brighton.
Colchester United lost 1-0 to Championship side Ipswich.
Chelmsford City beat Bishop’s Stortford 1-0.
Braintree Town lost 3-1 to Barnet and Billericay Town and Heybridge Swifts drew 3-3.
Meanwhile Tilbury lost 2-0 to Grays in the annual Stan Veness Trophy.
The charity match raised money for the family of former Tilbury player Kevin Malthouse who was killed in a stabbing incident earlier this year.