The club wrote on Twitter: “It is with immense sadness that we announce the passing of Ugo Ehiogu, our Under-23 coach.”
The former England international footballer had been taken to hospital after suffering a cardiac arrest at Tottenham Hotspur’s training ground on Thursday.
He had been working as the Premier League club’s under-23s coach.
The former Aston Villa and Middlesbrough defender collapsed and was treated at the scene before being taken to hospital by ambulance.
His wife Gemma and son had rushed to the hospital to be with him.
However the 44-year-old passed away in the early hours of Friday morning.
Ehiogu had been at Spurs since 2014, but began his career as a trainee at West Bromwich Albion before moving to Aston Villa in 1991.
He went on to feature in more than 300 matches for Villa over a nine-year spell, winning the League Cup in 1996.
After joining Middlesbrough for a then club-record £8m fee later that year, he won the same trophy with the northeast club in 2004.
Capped four times by England, he also played for West Brom, Leeds, Rangers and Sheffield United before retiring in 2009.